Identification of single-nucleotide polymorphisms of the ITGA2 integrin gene and their association with platelets in patients with arterial hypertension

Nazirova V.B., Guliev F.A., Gafarov I.A.

ABSTRACT

Arterial hypertension (AH) is one of the most socially significant pathologies associated with human nuclear genome mutations. The aim was to study the polymorphisms of the ITGA2B gene and its association with platelet parameters among Azerbaijanis with AH.

Methods. The study included 76 patients with AH (main group) and 24 patients without this pathology (control group). The main group was divided into 3 subgroups: group I— 29 patients with AH, group II— 23 patients with AH and coronary heart disease (CHD), group III— 24 patients with AH, CHD  and type 2 diabetes mellitus (DM). The analysis of platelets was carried out using hematologic analyzer, the polymorphism of ITGA2 gene— using mass spectrometry (MALDI-TOF).

Results. The prevalence of C/C, T/C and T/T genotypes of the ITGA2 gene was 69.0%, 17.2% and 13.8% in patients with AH; 65.2%, 21.7% and 13.0% in patients with AH and CHD, respectively; 62.5%, 29.2% and 8.3% in patients with AH, CHD and type 2 DM. The prevalence of the T allele among patients with AH was 31.0%, among patients with AH and CHD— 34.8%, and among patients with AH, CHD, and type 2 DM— 37.5%. The highest level of platelet count (PLT), platelet distribution width (PDW) and platelet-large cell ratio (P-LCR) were determined in group III, and the highest level of mean platelet volume (MPV) was seen in group II. The highest PLT was observed in T/T genotype carriers from group III; MPV in T/T genotype carriers from group I; PDW in T/T genotype carriers from group III; PCT in T/T genotype carriers from group III; P-LCR in T/T genotype carriers from group I.

Conclusions. According to the results obtained, the highest level of PLT, PDW and P-LCR were detected in patients with AH, CHD and DM-2, and MPV— in patients with AH and CHD. Marked changes in platelet parameters were noted in carriers of T/T and T/C  genotypes. The prevalence of the C/C, T/C, and T/T genotypes of the ITGA2 gene was 69.0%, 17.2%, and 13.8% in patients with AH; 65.2%, 21.7%, and 13.0% in patients with AH and CHD; and 62.5%, 29.2%, and 8.3% in patients with AH, CHD, and DM-2, respectively. Studies with larger samples are needed to confirm the results.

Keywords: arterial hypertension, ITGA2 gene, genotypes, allele, platelet parameters.

For citation:

Nazirova V.B.,  Guliev F.A., Gafarov I.A. Identification of single-nucleotide polymorphisms of ITGA2 integrin gene and their association with platelets in patients with arterial hypertension. International Journal of Heart and Vascular Diseases. 2022. 10(36): 10-16. doi: 10.24412/2311-1623-2022-36-10-16